Figure 1.

Trauma intensity of TBI organotypic hippocampal slice cultures incubated with various concentrations of desflurane for 2 hours.

Note: After 2-hour incubation, cell death was stained as shown on fluorescence images. Trauma intensity of the different groups is shown in relation to trauma intensity of mechanical traumatized slices (the trauma no desflurane group was set as 1 or 100%) after an incubation with 5% CO₂, 21% O₂,74% N₂ for 2 hours. Significant differences to the trauma no desflurane group are marked with ^*. There was no significant reduction in trauma intensity at the groups with mechanical trauma (P > 0.05). In the group without TBI, a concentration of 2% desflurane (^*P = 0.033) and 4% desflurane (^*P =0.004) reduced the trauma intensity significantly in comparison to the group without desflurane and trauma. For each group an average of 80 with a minimum of 56 slices were analyzed.