Figure 2.

Trauma intensity of TBI organotypic hippocampal slice cultures incubated with various concentrations of desflurane for 24 hours.

Note: After 24-hour incubation, cell death was stained as shown fluorescence images. Trauma intensity of different groups is shown in relation to trauma intensity of traumatized slices (the trauma no desflurane group after 2 hours of incubation was set as 1) after an incubation with 5% CO₂, 21% O₂,74% N₂ for 2 hours. Significant differences compared with the positive control group after two hours of incubation (Trauma without desflurane) are marked with ^*. Important significant differences between the groups after 24 hour incubation are also marked with ^* on the top of the figure. After 24 hours of incubation the trauma intensity tripled as compared to the trauma intensity after 2 hours of incubation. In the group without TBI the increase of trauma intensity rises also from 2% desflurane (^*P = 0.000) to 6% desflurane (^*P = 0.000). For the trauma group a significant increase of trauma intensity was observed at 4% desflurane (^*P = 0.006) and 6% desflurane (^*P = 0.000). For each group an average of 73 with a minimum of 62 slices were analyzed.