Figure 3.

Trauma intensity of TBI organotypic hippocampal slice cultures incubated with concentration of 4% desflurane for 2, 24 and 72 hours.

Note: In summary on this figure slices were exposed to a concentration of 4% desflurane and to 5% CO₂, 21% O₂, 74% N₂ (trauma no desflurane) for 2–72 hours. Trauma intensity of the different groups is shown in relation to trauma intensity of traumatized slices after an incubation without desflurane for 2 hours. Significant differences compared with the trauma no desflurane after two hours of incubation are marked with ^*. After 2 hours of incubation, there was no significant difference between the trauma no desflurane group and the trauma group with 4% desflurane (P = 0.273). After 24 hours, an increase to the double trauma intensity at the trauma group without desflurane was observed. Incubating with 4% desflurane after trauma led to an increase of trauma intensity to the four-fold. Finally after 72 hours the trauma intensity represents without desflurane and with 4% desflurane in the trauma group. For each group an average of 94 with a minimum of 73 slices were analyzed.