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USEARCH version 7 (blue) and 8 (orange) and VSEARCH (black) was run using the uchime_denovo (A, B) and the uchime_ref (C, D) commands on simulated Illumina data based on the SILVA database that has either been clustered with a 97% identity threshold (using the cluster_fast command in USEARCH) (A, C) or dereplicated (using the derep_fulllength command in VSEARCH) (B, D).
