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. 2016 Oct 24;7:455. doi: 10.3389/fimmu.2016.00455

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Copyright © 2016 Balakrishnan, Schnare and Chakravortty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial permeability-increasing protein is expressed in human monocyte/macrophages. Human or mouse monocytes/macrophages were exposed to indicated experimental conditions. Total RNA was extracted from (A) human and mouse monocyte and macrophage cell lines, (B) human peripheral blood monocytes (hPBMCs) and BPI expression were analyzed by semiquantitative RT-PCR. PCR products were purified and sent for sequencing for further confirmation. (C) Human PBMCs were PFA fixed and stained for BPI (red), CD11b (green), and nuclei (blue) and visualized by confocal microscopy.