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. 2016 Oct 24;7:455. doi: 10.3389/fimmu.2016.00455

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Copyright © 2016 Balakrishnan, Schnare and Chakravortty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Regulation of BPI expression in human monocytes/macrophages. U937 monocytes/macrophages were exposed to indicated inflammatory stimuli/pathogen. (A,B) Total RNA was isolated and BPI expression was investigated by real-time PCR [n = 5 (SD)]. (C) BPI expression was checked by flow cytometry [n = 3 (SD)]. (D) Total protein was isolated from U937 macrophage, and BPI levels were analyzed by western blot (n = 4). Bottom: quantitative evaluation of the relative BPI expression normalized to β-actin intensity. (E) Cells were PFA fixed and stained for BPI (red) and nuclei (blue). Cells were imaged by confocal microscopy (n = 6). Key: ***p < 0.001, **p < 0.005, *p < 0.05; ns, not significant.