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. 2016 Oct 24;7:455. doi: 10.3389/fimmu.2016.00455

Figure 3.

Figure 3

Human macrophages expressed BPI and kills intracellular bacteria. (A) Fold proliferation and percentage survival of bacteria after knocking down of BPI in U937 macrophages. Statistical significance was calculated with respect to untransfected control [n = 5 (SD)]. (B) Fold proliferation of STM after knocking down of BPI in human PBMCs-derived macrophages. Statistical significance was calculated with respect to scrambled dsRNA-transfected control [n = 6 (SD)]. (C) Total protein was isolated from BPI dsRNA-transfected and untransfected control U937 macrophages, and BPI levels were checked by western blot (n = 3). (D) RAW 264.7 macrophages were transfected with either pcDNA empty vector (pcDNA EV) or pcDNA carrying the expression sequence of human BPI (pcDNA hBPI). Twenty-four hours post-transfection, the cells were infected with STM and bacterial proliferation was quantified [n = 4 (SD)]. (E) Total protein was isolated from pcDNA EV-transfected and pcDNA hBPI-transfected RAW 264.7 macrophages, and BPI levels were quantified by western blot (n = 3). Key: ***p < 0.001, **p < 0.005, *p < 0.05; ns, not significant.