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. 2016 Oct 24;7:455. doi: 10.3389/fimmu.2016.00455

Figure 4.

Figure 4

BPI enhances bacterial uptake in human macrophages. (A) U937 macrophages were infected with STM–GFP (green) at an MOI of 10. Thirty minutes post-infection, cells were fixed with paraformaldehyde and stained for BPI (red) and nuclei (blue) (n = 4 experiments). (B,D) Percentage phagocytosis of bacteria after knocking down of BPI in U937 macrophages. U937 macrophages were infected with the indicated bacteria at an MOI of 10. Bacterial entry was quantified by plating cell lysates after 30 min post-infection [n = 6 (SD)]. For experiments with Salmonella Typhi (STY), percentage phagocytosis of BPI dsRNA transfected cells was compared to untransfected control and was normalized to STY (WT). (C) Percentage phagocytosis of STM in hPBMC-derived macrophages. Statistical significance was calculated with respect to scrambled dsRNA-transfected control [n = 6 (SD)]. Key: ***p < 0.001, **p < 0.005, *p < 0.05; ns, not significant.