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. 2016 Oct 24;7:455. doi: 10.3389/fimmu.2016.00455

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Copyright © 2016 Balakrishnan, Schnare and Chakravortty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BPI interaction with Gram-negative bacteria inside macrophages. (A) U937 macrophages were infected with GFP-tagged (green) Gram-negative bacteria [STM14028, PFA fixed STM, E. coli DH5α, and Shigella flexneri (SHG)] at an MOI of 50. Two hours post-infection, cells were fixed with PFA and stained for BPI (red) and nuclei (blue). White arrows indicate GFP-positive bacteria. The boxed area in the SHG infected set is magnified to view BPI around the bacteria (n = 3). (B) Quantification of colocalization of BPI with the bacteria. (C) Quantification of MFI of BPI at ROI was done by the Zen Blue edition software provided by Zeiss [n = 3 (SD)]. Key: ***p < 0.001, **p < 0.005, *p < 0.05.

BPI interaction with Gram-negative bacteria inside macrophages. (A) U937 macrophages were infected with GFP-tagged (green) Gram-negative bacteria [STM14028, PFA fixed STM, E. coli DH5α, and Shigella flexneri (SHG)] at an MOI of 50. Two hours post-infection, cells were fixed with PFA and stained for BPI (red) and nuclei (blue). White arrows indicate GFP-positive bacteria. The boxed area in the SHG infected set is magnified to view BPI around the bacteria (n = 3). (B) Quantification of colocalization of BPI with the bacteria. (C) Quantification of MFI of BPI at ROI was done by the Zen Blue edition software provided by Zeiss [n = 3 (SD)]. Key: ***p < 0.001, **p < 0.005, *p < 0.05.