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. 2016 Oct 24;6:35854. doi: 10.1038/srep35854

Figure 3. CDC25C promotes tumor cell dedifferentiation and growth.

Figure 3

(A) Differential growth of 5926 (predominantly basal cells) and 277 (predominantly fully differentiated cells) cell lines in vitro. Cell count was manually determined using a hemocytometer for four biological replicates per sample. Relative cell count was determined by normalization to day 0 values. (B) Western blot analysis of CDC25C protein expression in parental 5926 and 277 cell lines and in 5926 cells after treatment with siRNA against CDC25C (siCDC25C) or a scrambled control (Scr). β-actin protein level served as a loading control. (C) Flow cytometric analysis of bladder tumor cell subpopulations after treatment of 5926 and 277 cells with siRNA against CDC25C or a scrambled control. TP, triple-positive (basal); DP, double-positive (intermediate); SP, single-positive (differentiated); TN, triple-negative (fully differentiated). (D) Effect of CDC25C gene suppression on 5926 cell growth. Relative cell count was determined by normalization to day 0 values. Error bars denote standard error of mean. P-values were determined using 2-tailed Student’s t-test. Asterisks denote P-values ≤ 0.05.