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. 2016 Oct 24;6:35970. doi: 10.1038/srep35970

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Copyright © 2016, The Author(s)

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(a,b) The expression of Ampd1 in different tissues. Ampd1 was predominantly expressed in the muscle as determined with real-time PCR (a) and Western blot (b) in E18.5 WT mice. (c,d) The validation of Ampd1 disruption. Ampd1 mRNA (as compared with WT, ****P < 0.0001; **P < 0.001, One-way ANNOVA followed with Bonferroni’s multiple comparisons test) and protein (d) were undetectable in the hind-leg muscle of E18.5 Ampd1^tm1a/tm1a mice. Relative mRNA level was normalized to β-actin.