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. 2016 Oct 24;6:35970. doi: 10.1038/srep35970

Figure 4. The knockout-first cassette in Ampd1 tm1a allele influences the neighboring genes expression.

Figure 4

(a) Real time PCR indicated that Man1a2 was significant down-regulated in the brain, heart, kidney, lung and muscle; (b) Nras was slight down-regulated in the liver and the muscle of Ampd1tm1a/tm1a mice (n = 5/each genotype. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-way ANNOVA followed with Bonferroni’s multiple comparisons test). (c) Schematic diagram of Man1a2, Nras and Ampd1 on chromosome 3. Nras is 6 kb from Ampd1 gene, whereas Man1a2 is 2.4 Mb away from Ampd1on the other strand. Arrowheads indicate orientation (left, negative strand; right, positive strand). (d) Relative mRNA expression of Ampd1, Man1a2, Nras in the muscle from different mice allele (As compared to WT, n = 5/each genotype, **P < 0.01, ****P < 0.0001, one-way ANNOVA followed with Bonferroni’s multiple comparisons test).