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siRNA screening for genes required for DNA damage–induced neural cell death. A, Schematic diagram of lentiviral siRNA library screening. B, Fragments of siRNA inserts were amplified from lentivirus-infected, MNNG-treated surviving neural stem cells and reference control cells (lentivirus infection but no MNNG treatment) by two rounds PCR. C, Scatterplot analysis of siRNA inserts enriched in neural stem cells treated by MNNG.
