Figure 5.

CCR3 deletion or inhibition protects neurons against OGD-induced excitotoxicity. A, Primary cultured cortical neurons were isolated from CCR3 KO and WT embryos at embryonic day 15. Neurons (14 days in vitro) were pretreated with or without CCR3 inhibitor SB328437 at the indicated dose before being subjected to OGD treatment for 90 min. Twenty-four hours after OGD, neurons were stained with Hoechst 33342 and propidium iodide (PI). Dead neurons were scored as red cells with condensed or fragmented nuclei. Scale bar =100 µm. B, Quantification of cell death in different treatments. Experiments were performed at least three times. Data represent the mean ± SEM. * p<0.05, one-way ANOVA with Tukey–Kramer test.