Table 1.
Summary of EIA, hemi-nested and qRT-PCR results for samples with a low viral load which were genotyped by hemi-nested assay amplification and sequencing.
| Sample # | EIA | Genotype by hemi nested PCR | NSP3 Ct value | NSP3 copy number | VP7 genotype by nested PCR and sequencing | VP4 genotype by nested PCR and sequencing |
|---|---|---|---|---|---|---|
| 1 | 0.063 | GNT P[NT] | 41.6 | ≤1 | G1 | P[8] |
| 2 | 0.049 | GNT P[NT] | 39.3 | ≤1 | Negative | Negative |
| 3 | 0.048 | G1 P[NT] | 39.3 | ≤1 | G1 | P[8] |
| 4 | 0.055 | GNTP[NT] | 38.6 | ≤1 | G1 | P[8] |
| 5 | 0.049 | GNT P[NT] | 38.6 | ≤1 | G1 | P[8] |
| 6 | 0.052 | GNT P[NT] | 38.2 | ≤1 | G3 | P[8] |
| 7 | 0.060 | GNT P[NT] | 36.7 | 1.1 | G3 | Negative |
| 8 | 0.061 | G1 P[NT] | 39.8 | ≤1 | G1 | P[4] |
| 9 | 0.095 | GNT P[NT] | 38.2 | ≤1 | G1 | P[8] |
| 10 | 0.088 | GNT P[NT] | 36.5 | 1.2 | G1 | P[8] |
| 11 | 0.104 | GNT P[NT] | 40.8 | ≤1 | G3 | P[8] |
| 12 | 0.104 | GNT P[NT] | 33.0 | 13.0 | G1 | P[8] |
| 13 | 0.088 | GNT P[NT] | 38.7 | ≤1 | G1 | Negative |
| 14 | 0.086 | GNT P[NT] | 36.5 | 1.2 | G1 | P[8] |
| 15 | 0.099 | GNT P[NT] | 31.5 | 36.2 | G1 | P[8] |
| 16 | 0.093 | GNT P[NT] | 27.2 | 685.2 | G3 | P[8] |
| 17 | 0.056 | GNT P[NT] | 40.0 | ≤1 | Negative | P[8] |
| 18 | 0.056 | GNT P[NT] | 38.7 | ≤1 | G1 | P[8] |
| 19 | 0.052 | GNT P[NT] | 40.4 | ≤1 | Negative | P[8] |
| 20 | 0.060 | GNT P[NT] | 37.0 | ≤1 | G9 | P[8] |
| 21 | 0.059 | GNT P[NT] | 36.3 | 1.4 | G3 | P[8] |
| 22 | 0.058 | GNT P[NT] | 34.5 | 4.7 | G3 | P[6] |
| 23 | 0.057 | GNT P[NT] | 40.4 | ≤1 | G3 | P[8] |
| 24 | 0.056 | GNT P[NT] | 29.4 | 161.1 | G1 | P[8] |
| 25 | 0.053 | GNT P[NT] | 37.3 | ≤1 | G1 | P[8] |
| 26 | 0.052 | GNT P[NT] | 38.4 | ≤1 | G1 | P[8] |
| 27 | 0.055 | GNT P[NT] | 38.8 | ≤1 | G1 | P[8] |
| 28 | 0.059 | GNT P[NT] | 40.0 | ≤1 | G1 | P[8] |
| 29 | 0.060 | GNT P[NT] | 36.2 | 1.5 | G1 | P[8] |
| 30 | 0.057 | GNT P[NT] | 35.9 | 1.9 | Negative | P[6] |
| 31 | 0.054 | GNT P[NT] | 32.3 | 21.1 | G1 | P[8] |
| 32 | 0.061 | GNT P[NT] | 31.6 | 33.8 | G1 | P[8] |
| 33 | 0.053 | GNT P[NT] | 30.4 | 80.3 | G3 | P[8] |
| 34 | 0.052 | GNT P[NT] | 35.3 | 2.8 | G1 | P[8] |
| 35 | 0.054 | GNT P[NT] | 35.6 | 2.3 | Negative | Negative |
| 36 | 0.054 | GNT P[NT] | 39.8 | ≤ 1 | G3 | P[8] |
| 37 | 0.053 | GNT P[NT] | 34.2 | 5.7 | G1 | P[8] |
| 38 | 0.065 | GNT P[NT] | 38.2 | ≤1 | G1 | P[8] |
| 39 | 0.097 | GNT P[NT] | 35.5 | 2.4 | G1 | P[8] |
| 40 | 0.077 | GNT P[NT] | 39.5 | ≤1 | G1 | P[8] |
| 41 | 0.055 | GNT P[NT] | 38.9 | ≤1 | G1 | P[8] |
| 42 | 0.046 | GNT P[NT] | 39.4 | ≤1 | Negative | P[8] |
| 43 | 0.057 | GNT P[NT] | 39.2 | ≤1 | G1 | P[8] |
| 44 | 0.093 | GNT P[NT] | 30.4 | 80.4 | G3 | P[8] |
| 45 | 0.073 | GNT P[NT] | 35.2 | 2.9 | G1 | P[8] |
| 46 | 0.090 | GNT P[NT] | 36.5 | 1.2 | G1 | P[8] |
| 47 | 0.088 | GNT P[NT] | 40.1 | ≤1 | Negative | P[8] |
| 48 | 0.077 | GNT P[NT] | 33.5 | 9.3 | Negative | P[4] |
| 49 | 0.074 | GNT P[NT] | 33.7 | 8.3 | G1 | P[8] |
| 50 | 0.092 | GNT P[NT] | 31.6 | 35.3 | G3 | P[6] |
| 51 | 0.149 | GNT P[NT] | 37.8 | ≤1 | G1 | P[8] |
| 52 | 0.086 | GNT P[NT] | 33.3 | 11.0 | G1 | P[8] |
| 53 | 0.074 | GNT P[NT] | 34.0 | 6.7 | Negative | Negative |
| 54 | 0.069 | GNT P[NT] | 32.0 | 26.4 | G3 | P[8] |
| 55 | 0.068 | G2 P[NT] | 27.0 | 804.7 | G2 | P[8] |
| 56 | 0.054 | G2 P[NT] | 44.0 | ≤1 | G1 | P[8] |
| 57 | 0.059 | GNT P[NT] | 39.0 | ≤1 | G3 | P[8] |
| 58 | 0.057 | GNT P[NT] | 30.0 | 103.5 | G8 | P[8] |
| 59 | 0.068 | GNT P[NT] | 32.0 | 26.4 | G1 | P[8] |
| 60 | 0.072 | GNT P[NT] | 35.0 | 3.4 | G2 | P[4] |
| 61 | 0.048 | GNT P[NT] | 41.0 | ≤1 | G1 | P[8] |
| 62 | 0.080 | GNT P[NT] | 43.0 | ≤1 | G3 | P[8] |
| 63 | 0.080 | GNT P[NT] | 35.0 | 3.4 | G1 | P[8] |
| 64 | 0.080 | GNT P[NT] | 41.0 | ≤1 | G1 | P[8] |
NT = non-typeable