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. Author manuscript; available in PMC: 2016 Oct 24.
Published in final edited form as: J Virol Methods. 2016 Jul 13;236:41–46. doi: 10.1016/j.jviromet.2016.07.007

Table 2.

Hemi-nested and qRT-PCR results for well-characterized samples which were genotyped by hemi-nested assay amplification and sequencing.

Sample # Genotype by hemi nested PCR or sequencing NSP3 Ct value NSP3 copy number VP7 genotype by nested PCR and sequencing VP4 genotype by nested PCR and sequencing
65 G1P[8] 29.0 2.1E + 02 G1 P[8]
66 G12P[6] 28.9 2.2E + 02 G12 P[6]
67 G12P[6] 13.0 1.2E + 07 G12 P[6]
68 G12P[6] 21.0 4.0E + 04 G12 P[6]
69 G9P[8] 17.2 6.5E + 05 G9 P[8]
70 G9P[6] 22.0 2.5E + 04 G9 P[6]
71 G9P[8] 22.3 2.0E + 04 G9 P[8]
72 G2P[4] 14.3 4.7E + 06 G2 P[4]
73 G2P[4] 27.0 8.0E + 02 G2 P[4]
74 G2P[4] 19.1 1.8E + 05 G2 P[4]
75 G9P[8] 18.2 3.3E + 05 G9 P[8]
76 G1P[8] 17.8 4.3E + 05 G1 P[8]
77 G9P[8] 13.8 6.7E + 06 G9 P[8]
78 G1P[8] 19.1 1.8E + 05 G1 P[8]
79 G3P[6] 16.8 8.6E + 05 G3 P[6]
80 G12P[8] 17.2 6.5E + 05 G12 P[8]
81 G4P[6] 21.2 4.2E + 04 G4 P[6]
82 G4P[6] 20.2 8.4E + 04 G4 P[6]
83 G4P[6] 22.5 1.7E + 04 G4 P[6]
84 G1P[8] 27.1 7.5E + 02 G1 P[8]
85 G3P[6] 14.5 4.1E + 06 G3 P[6]
86 G3P[6] 15.2 2.6E + 06 G3 P[6]
87 G2P[4] 17.1 7.0E + 05 G2 P[4]
88 G12P[8] 14.1 5.4E + 06 G12 P[8]
89 G12P[8] 15.5 2.1E + 06 G12 P[8]
90 G3P[6] 14.2 5.1E + 06 G3 P[6]
91 G3P[6] 14.0 5.8E + 06 G3 P[6]