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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Mol Biol. 2016;1481:183–196. doi: 10.1007/978-1-4939-6393-5_17

Table 1.

Antibodies used in this study.

Antibody Source/Isotype/clone/cat. no. Dilution
CD31-APC MiltenyiBiotec, mouse IgG1, Clone: AC128 1:50
CD34-FITC Miltenyi Biotec, mouse IgG2a, Clone: AC136 1:50
CD31-FITC Miltenyi Biotec, mouse IgG1, Clone: AC390 1:50
VE-cadherin Santa Cruz, mouse IgG1, Clone: F-8 sc9989 1:100
KDR Santa Cruz, mouse IgG1, Clone: A-3 sc6251 1:200
vWF Dako, rabbit IgG, Cat. no: A008202-5 1:500
Brachyury R&D Systems, Gotat IgG, Clone: AF2085 1:100
Oct4 Santa Cruz, Mouse IgG2b, sc5279 1:100
Secondary Antibody Alexa 488 Chicken anti-Gt IgG/A-21467 1:1,000
Secondary Antibody Alexa 488 Goat anti-Ms IgG1/A-21121 1:1,000
Secondary Antibody Alexa 488 Goat anti-Rb IgG/A-11008 1:1,000
Secondary Antibody Alexa 594 Goat anti-Ms IgG2b/A-21145 1:1,000
Secondary Antibody Alexa 594 Goat anti-Rb IgG/A-11012 1:1,000
Secondary Antibody Alexa 647 Goat anti-Ms IgG2b/A-21242 1:1,000
Secondary Antibody Alexa 647 Goat anti-Rb IgG/A-21244 1:1,000