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. 2016 May 26;311(3):G396–G411. doi: 10.1152/ajpgi.00027.2016

Fig. 1.

Fig. 1.

Yes-associated protein (YAP) antagonizes hepatocyte adherens junction stability in mouse livers. A: serum total bilirubin levels of wild type (WT) mice, Yap-KO (KO) mice, and Yap-Tg (Tg) mice. Bars represent mean ± SE (n = 3 mice from each genotype). *P < 0.05, compared with WT by one-way ANOVA and Fisher's least significant difference posttest. B: ZO-1 staining of WT, Yap-KO, and Yap-Tg livers. C: E-cadherin staining of WT, Yap-KO, and Yap-Tg livers. Note more E-cadherin staining on the hepatocyte membrane of Yap-Tg livers. D: transmission electron microscopy images of WT, Yap-KO, and Yap-Tg livers showed the length of adherens junctions (indicated by yellow dash lines) surrounding bile canaliculi positively correlated with YAP levels. The electron dense zones demarcating the adherens junctions in Yap-Tg livers extended for a longer distance along the membrane and penetrated further into the cytoplasm. H, hepatocyte; C, bile canaliculi. E: E-cadherin mRNA levels in WT, Yap-KO, and Yap-Tg livers. Bars represent mean ± SE (n = 3 mice from each genotype). F: E-cadherin protein levels WT, Yap-KO, and Yap-Tg livers. G: serum total bilirubin levels of WT and KO mice 5 days after BDL. Bars represent mean ± SE (n = 5 mice from each genotype). *P < 0.05, compared with WT by unpaired t-test.