Fig. 5.

NF-κB activation is unaltered by the decrease in hepatocyte autophagy. A: hepatic cytosolic protein fractions from individual control (Con) (Cre −) and knockout (KO) (Cre +) mice untreated or treated with LPS for the indicated hours and probed for IκBα, the nuclear loading control NOPP140, and the cytosolic protein loading control GAPDH. B: nuclear protein fractions from the same mice probed for the p65 and p50 subunits of NF-κB, NOPP140, and GAPDH. C–E: relative protein levels of IκBα, p65, and p50 proteins, respectively, by densitometric scanning of immunoblots (n = 4). F–H: relative Traf2, Birc2, and Birc3 mRNA levels, respectively, determined by qRT-PCR in Con and KO mice untreated, or treated with LPS for hours indicated. Values are means ± SE.