Figure 1. Autophagic flux is reduced in aged primary human fibroblasts.

(A) Correlation of age and LC3-II protein levels in primary dermal human fibroblasts from differently aged donors. AU: artificial units. (B) Immunostaining of LC3B protein in cells from young and old donors after Bafilomycin treatment. In the bottom row cells stained only with secondary antibody (Cy3) and DAPI are shown. Total signal strength (C) and the number of LC3 positive punctae in a defined area (D) were quantified.