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. 2016 Aug 28;8(9):1876–1890. doi: 10.18632/aging.101018

Figure 3. Autophagy in Cryptochrome-deficient MEFs.

Figure 3

(A) Circadian accumulation of Bmal1 mRNA (squares) and Per2 mRNA (circles) in synchronised wild type and Cry1/2−/− MEFs. (B) Bmal1, Per2, Nr1d1 and Per1 mRNA levels in Cry1/2−/− MEFs normalised to the corresponding values in wild type MEFs. Data are shown as mean values ± SEM, n=8. (C) Autophagic flux in wild type MEFs (white) and Cry1/2−/− MEFs (black) determined by quantification of LC3-II protein levels in the absence or presence of bafilomycin (Bafilo).