Figure 4. Genetic disruption of PPARβ/δ attenuates laser-induced CNV.

(A) Choroidal flat-mounts were prepared from 18–20 month-old laser-induced CNV mice (Pparβ/δ^+/+, n = 10 eyes, 40 lesions; Pparβ/δ^−/−, n=5 eyes, 20 lesions) and stained with isolectin GS-IB₄ (ON, optic nerve). Representative images from three eyes/genotype are shown to demonstrate individual and merged CNV lesions. Dotted and solid line circles demarcate lesions and optic nerves, respectively. (B) Distribution of number of eyes with individual versus merged lesions in Pparβ/δ^+/+ and Pparβ/δ^−/−. (C) Relative lesion area/animal was measured using ImageJ (mean and S.E.M.; *p < 0.01, two tailed t-test). (D) Relative lesion volume/animal (mean and S.E.M. for each group; *p < 0.05, two tailed t-test).