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. 2016 Sep 8;8(9):1952–1971. doi: 10.18632/aging.101031

Figure 7. Antagonism of PPARβ/δ pathway attenuates CNV.

Figure 7

(A) Choroidal flat-mounts were prepared from 12-13 month-old C57BL/6J (Pparβ/δ+/+) mice subjected to laser CNV and treated with vehicle control (1% DMSO in saline), GW0742 (0.5 mg/kg/day, i.p.), and GSK0660 (1 m/kg/day, i.p.) and stained with isolectin-GS-IB4 (n=10 eyes/group, 40 lesions; ON, optic nerve). Representative images from three eyes/treatment are shown to demonstrate individual and merged CNV lesions (dotted and solid line circles demarcate lesions and ONs, respectively). (B) Distribution of number of eyes with individual versus merged lesions in vehicle control, GW0742 and GSK0660. (C) Relative lesion area/animal was measured using ImageJ (n=10 eyes per group, mean and S.E.M.; *p < 0.01, one way ANOVA, Tukey's multiple comparisons test). (D) Relative lesion volume/animal (n=10 eyes per group, mean and S.E.M. for each group; *p < 0.05. ns: not significant, one way ANOVA, Tukey's multiple comparisons test).