Figure 5.
Efficacy study after intravenous administration of VSV-mIFN-NIS into SCID mice with human KAS6/1 myeloma xenografts. (a) Single-photon emission computed tomography/computed tomography (SPECT/CT) imaging showing sodium iodide symporter (NIS) expression and positive isotope uptake in tumor (circled) from mice treated with VSV but not saline, (b) corresponding dosimetric measurements of NIS-mediated isotope uptake, (c) tumor volume (mean ± SEM), and (d) survival curve of treated animals. Viral load was measured by qRT-PCR for VSV-N RNA or infectious virus in (e, h) tumor, (f, i) brain, and (g, j) spleen of animals at necropsy when the animals were euthanized for tumor burden or at the end of the study. Horizontal bars represent group medians. Dotted lines represent the limit of detection: 190 TCID50/g for IVR, 103 copies/μg RNA for qRT-PCR.