Table 1.
Multiplex cytokine assay of plasma from mice 3 or 24 hours after intravenous VSV-mIFNβ-NIS virus administration
| Dose (TCID50) | Time point (hr) | IL-1β (pg/ml) | IL-10 (pg/ml) | IL-6 (pg/ml) | IL-12 (pg/ml) | GM-CSF (pg/ml) | IL-5 (pg/ml) | IFN-γ (pg/ml) | TNF-α (pg/ml) | IL-2 (pg/ml) | IL-4 (pg/ml) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Saline | 24 | <5 | <10 | <5 | 96.6 ± 24 | 1.4 ± 0.7 | 19.1 | <5 | 9.8 ± 3.5 | <5 | <20 |
| VSV (1 × 108) | 3 | <5 | <10 | 50.7 ± 30.5 | 83.4 ± 45.2 | 1.5 ± 0.6 | 23.9 ± 35.5 | <5 | 24.5 ± 13.4 | <5 | <20 |
| 24 | <5 | <10 | <5 | 93.1 ± 85.2 | 1.7 ± 0.6 | 6.5 ± 12.0 | <5 | 17.5 ± 15.9 | <5 | <20 | |
| VSV (3 × 108) | 3 | 12.0 ± 8.4 | <10 | <5 | 114.9 ± 25.6 | 3.3 ± 1.3 | 11.9 ± 8.7 | <5 | 54.9 ± 45.2 | 8.5 ± 5.2 | <20 |
| 24 | 10.1 ± 5.7 | <10 | <5 | 134.6 ± 33.8 | 1.7 ± 0.6 | 2.1 ± 2.3 | <5 | 23.7 ± 17.7 | <5 | <20 | |
| VSV (1 × 109) | 3 | 8.2 ± 6.0 | <10 | 28.5 ± 26.8 | 92.3 ± 39.1 | 2.7 ± 0.8 | 7.1 ± 8.0 | <5 | 25.3 ± 13.2 | 6 ± 0.5 | <20 |
| 24 | 16.5 ± 10.2 | <10 | 125.2 ± 9.9 | 137.2 ± 30.3 | 4.5 ± 2.5 | 62.3 ± 48.7 | <5 | 51.8 ± 31.7 | 10.9 ± 5.0 | <20 |
GM-CSF, granulocyte-macrophage colony-stimulating factor; IFN, interferon; IL, interleukin; TCID50, 50% tissue culture infective dose; TNF, tumor necrosis factor; VSV, vesicular stomatitis virus.