Table 2.

Quantitative RT-PCR for VSV-N RNA and infectious virus recovered from major organs in mice bearing 5TGM1 tumors after treatment with one dose of 10⁵, 10⁶, 10⁷, or 10⁸ TCID₅₀ of VSV-mIFNβ-NIS

	Organ	Saline	105 TCID50	106 TCID50	107 TCID50	108 TCID50
qRT-PCR for VSV-N (copy number/μg RNA)	Tumor	<103	<103	2.08 × 103	1.07 × 107	1.92 × 108
		<103	6.22 × 106	2.14 × 107	2.23 × 107	5.06 × 108
		<103	1.44 × 106	<103	<103	2.19 × 108
	Brain	<103	<103	<103	<103	<103
		<103	<103	<103	<103	<103
		<103	<103	<103	<103	<103
	Spinal cord	<103	<103	<103	1.69 × 103	<103
		<103	<103	<103	<103	4.63 × 105
		<103	<103	<103	<103	1.88 × 103
	Spleen	<103	<103	5.13 × 103	2.2 × 104	5.9 × 105
		<103	<103	1.09 × 104	1.6 × 104	7.37 × 105
		<103	<103	7.96 × 103	5.49 × 103	8.51 × 105
	Liver	<103	<103	<103	1.8 × 103	4.81 × 103
		<103	<103	<103	<103	6.15 × 103
		<103	<103	<103	1 × 103	1.27 × 104
	Ovary	<103	<103	<103	1.8 × 103	4.81 × 103
		<103	<103	<103	<103	6.15 × 103
		<103	<103	<103	1 × 103	1.27 × 104
Infectious virus recovery (TCID50/g tissue)	Tumor	<190	3.39 × 107	<190	3.56 × 107	4.31 × 107
		<190	<190	1.9 × 105	<190	1.9 × 107
		<190	<190	<190	<190	<190
	Brain	<190	<190	<190	<190	<190
		<190	<190	<190	<190	<190
		<190	<190	<190	<190	<190
	Spleen	<190	<190	<190	1.9 × 103	<190
		<190	<190	<190	<190	<190
		<190	<190	<190	<190	<190

Note: Limit of detection for qRT-PCR: >10³ and >190 for infectious virus recovery. n = 3 mice per group.

TCID₅₀, 50% tissue culture infective dose; VSV-N, vesicular stomatitis virus nucleocapsid.