FIGURE 11.

A, effect of function-blocking mAbs on HT-1080 cells pre-spread on 50K. Cells were allowed to spread on 50K for 1 h and then exposed to the indicated anti-α5 (JBS5, 16, P1D6, or SNAKA52) or anti-β1 (13, 4B4, AIIB2, or Lia1/2) mAbs for 1 h. The percentage of spread cells was then quantitated. The non-function-blocking anti-α5 mAb 11 and anti-β1 mAb K20 were used as controls. B, effect of the same mAbs in an assay in which the mAbs were added to the cells before spreading on 50K. Similar results were obtained in three separate experiments.