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Measurement of ROS. The rates of production in ROS from two affected matrilineal relatives and two control individuals were analyzed by a BD-LSR II flow cytometer system with or without H2O2 stimulation. The relative ratio of intensity (stimulated versus unstimulated with H2O2) was calculated. The average of three determinations for each cell line is shown. The graph details and symbols are explained in the legend for Fig. 4.
