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Measurement of cellular and mitochondrial ATP levels using a bioluminescence assay. Cells were incubated with 10 mm glucose or 5 mm 2-deoxy-d-glucose plus 5 mm pyruvate to determine ATP generation under mitochondrial ATP synthesis. Average rates of ATP level per cell line and are shown. A, ATP level in total cells. B, ATP level in mitochondria. Four to five determinations were made for each cell line. The graph details and symbols are explained in the legend for Fig. 4.
