FIGURE 9.

Mitochondrial membrane potential analysis. The mitochondrial membrane potential was measured in two mutant and two control cell lines using a fluorescence probe JC-10 assay system. The ratio of fluorescence intensities Ex/Em = 490/590 and 490/530 nm (FL590/FL530) were recorded to delineate the ΔΨm level of each sample. The relative ratios of FL590/FL530 geometric mean between mutant and control cell lines were calculated to reflect the level of ΔΨm. Shown is the relative ratio of JC-10 fluorescence intensities at Ex/Em = 490/530 and 490/590 nm in the absence (A) and presence (B) of 10 μm FCCP. The average of four to five determinations for each cell line is shown. The graph details and symbols are explained in the legend for Fig. 4.