FIGURE 3.
Effect of soluble VCAM-1 on the lateral mobility of α4β1in T cells. A, overlay semi-log distributions of D1–4 values for untreated cells or cells exposed to soluble VCAM-1-Fc in solution for 0–2, 2–5, and 5–10 min. B, percentage of immobile, slow, and fast α4β1 subpopulations for untreated or VCAM-1-exposed cells. C, diffusion coefficients of the total mobile population and for the slow and fast fractions of α4β1. D, anomalous α exponents for the slow and fast α4β1 subpopulations. Ten untreated cells (1939 trajectories), five cells stimulated with VCAM-1 for 0–2 min (787 trajectories), eight cells stimulated with VCAM-1 for 2–5 min (1582 trajectories), and 11 cells stimulated with VCAM-1 for 5–10 min (1550 trajectories) over three separate experiments. ns, not significant (p > 0.05); *, p < 0.05; **, p < 0.01.