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. 2016 Aug 1;291(40):21053–21062. doi: 10.1074/jbc.M116.733709

FIGURE 4.

FIGURE 4.

Effect of CXCL12 and VCAM-1 co-stimulation on the lateral mobility of α4β1in T-cells. A and B, data correspond to the first 0- to 2-min period of the different stimulation conditions. A, overlay semi-log distributions of D1–4 values for the indicated conditions. Data from the CXCL12 stimulation experiments (Fig. 1) are included to allow easier comparison of both conditions. B, percentage of immobile, slow, and fast α4β1 subpopulations. C and D, the same as A and B at the 2- to 5-min interval of stimulation. E and F, the same as in A and B at the 5- to 10-min interval of stimulation. 29 untreated cells (2382 trajectories), five cells co-stimulated with CXCL12 and VCAM-1 (260 trajectories) during 0–2 min, seven cells co-stimulated with CXCL12 and VCAM-1 (301 trajectories) during 2–5 min, and nine cells (587 trajectories) during 5–10 min over two separate experiments. ns, not significant (p > 0.05); *, p < 0.05; **, p < 0.01; ***, p < 0.001.