FIGURE 5.

NF90-NF45 complex is bound to endogenous pri-miR-7-1. WCEs from Huh7 cells transfected with indicated TARGET-tagged NF90 and NF45 were immunoprecipitated with anti-TARGET tag antibody. A, target-tagged NF90 and NF45 in the immunoprecipitates (IP) were detected by immunoblotting probed with anti-NF90, anti-NF45, anti-TARGET tag, and anti-GAPDH antibodies. 20% of WCEs was used as input. B, RNAs were isolated from precipitates and analyzed by qRT-PCR with specific primers for pri-miR-7-1 or β-actin. 4% of WCEs was used as input. RT− was negative control of reverse transcription. Data are expressed as the mean ± S.D. of triplicate PCRs with ranges and are representative of three independent experiments.