FIGURE 6.

Huwe1 knockdown increased the stability of Atoh1 in organ of Corti explants. A, organs of Corti were treated with proteasome inhibitor MG132 (10 μm) for 6 h. Atoh1 and β-actin (loading control) were quantified by Western blotting (IB) and densitometry (Atoh1 levels are shown below each lane). B, P1 organ of Corti was treated with the indicated siRNA (100 nm) for 72 h. Experiments were done in triplicate. Error bars indicate S.E. (n = 3 experiments). C, organs of Corti were treated with the indicated siRNA (100 nm) for 72 h. Huwe1, myosin VIIa, Atoh1, and HSC70 (loading control) were quantified by Western blotting, and densitometry (Huwe1, myosin VIIa, and Atoh1) levels are shown below each lane).