TABLE 2.
Analysis of Huwe1
Coomassie Blue-stained band of 450 kDa indicated in Fig. 1E was excised and subjected to mass spectrometric analysis to validate Huwe1 (shown in bold) as a binding partner of Atoh1.
| Gene symbol | Total peptide | Unique peptide | Average peptide score | Protein coverage |
|---|---|---|---|---|
| % | ||||
| HUWE1 | 279 | 122 | 3.5367 | 28.78 |
| PRKDC | 161 | 112 | 3.3479 | 25.48 |
| MAP1B | 133 | 78 | 3.7349 | 37.68 |
| DYNC1H1 | 87 | 75 | 3.2275 | 18.02 |
| KIF11 | 79 | 46 | 3.3049 | 37.97 |
| SRRM2 | 53 | 38 | 3.7266 | 20.75 |
| MACF1 | 51 | 50 | 3.2235 | 8.57 |
| MYCBP2 | 51 | 47 | 3.3464 | 13.64 |
| MDN1 | 41 | 37 | 3.3880 | 8.79 |
| HERC2 | 36 | 34 | 3.2990 | 9.08 |
| RNF213 | 32 | 30 | 3.0488 | 6.38 |
| UBR5 | 31 | 26 | 3.3120 | 12.54 |
| UTRN | 30 | 30 | 3.4022 | 10.81 |
| MGA | 29 | 27 | 3.6467 | 13.52 |
| AKAP9 | 26 | 26 | 2.9820 | 8.08 |
| PRRC2C | 26 | 21 | 3.2923 | 8.08 |
| CEP350 | 23 | 21 | 3.1575 | 11.08 |
| SON | 22 | 20 | 3.6486 | 9.66 |
| PLEC | 17 | 16 | 2.8815 | 3.86 |
| MAP1A | 15 | 14 | 3.2701 | 8.46 |
| DMXL1 | 13 | 12 | 3.1873 | 5.48 |
| TRRAP | 12 | 12 | 3.1620 | 3.91 |
| SACS | 12 | 12 | 2.5812 | 2.88 |
| GOLGB1 | 12 | 10 | 2.8166 | 3.28 |