TABLE 3.
Analysis to confirm immunoprecipitation of Atoh1
Coomassie Blue-stained band of 45 kDa indicated in Fig. 1E was excised and subjected to mass spectrometry. ATOH1 and CK1 isoforms are in bold.
| Gene symbol | Total peptide | Unique peptide | Average peptide score | Protein coverage |
|---|---|---|---|---|
| % | ||||
| TUBB2A | 159 | 40 | 3.0903 | 55.5 |
| ATOH1 | 155 | 19 | 2.1144 | 34.18 |
| TUBA1A | 112 | 34 | 3.1383 | 66.08 |
| RPL4 | 106 | 36 | 2.4767 | 53.63 |
| EEF1A1 | 103 | 28 | 2.6454 | 58.87 |
| TUFM | 72 | 38 | 3.2781 | 68.36 |
| RPL3 | 67 | 29 | 2.5399 | 45.66 |
| SSB | 65 | 30 | 2.8608 | 5.43 |
| RBMX | 65 | 27 | 2.5009 | 47.06 |
| ENO1 | 62 | 34 | 3.1674 | 58.29 |
| PSMC5 | 52 | 31 | 3.5295 | 64.29 |
| PSMC2 | 50 | 29 | 2.9925 | 63.74 |
| YARS2 | 11 | 8 | 3.3677 | 29.77 |
| TUBA1C | 7 | 11 | 3.6062 | 15.14 |
| SERPINH1 | 9 | 7 | 2.5123 | 20.81 |
| CSNK1D | 7 | 4 | 3.1615 | 12.5 |
| CSNK1E | 6 | 3 | 2.8233 | 9.86 |
| ACOX1 | 5 | 4 | 2.8799 | 10.30 |
| BCKDHA | 5 | 3 | 2.6629 | 9.44 |
| PRPS1 | 3 | 3 | 2.6642 | 18.46 |
| TUBB2B | 2 | 1 | 3.0318 | 2.70 |
| TUBA4A | 1 | 1 | 3.4258 | 31.31 |
| PRDX4 | 1 | 1 | 3.1432 | 7.01 |
| AAMP | 1 | 1 | 2.3377 | 2.53 |
| PDE4B | 1 | 1 | 2.2331 | 2.04 |