TABLE 4.
Summary of Atoh1 Phosphorylation
| Positiona | Atoh1b | Atoh1 + CK1c | Conservationd | Play a role in Atoh1 degradatione | Peptidef |
|---|---|---|---|---|---|
| 309 | x | x | N | No | DLSPSLPGGILQPVQEDNSK |
| 311 | x | N** | N/A | DLSPSLPGGILQPVQEDNSK | |
| 325 | x | Y | No | DLSPSLPGGILQPVQEDNSKTSPR | |
| 328 | x | Y | Low | DLSPSLPGGILQPVQEDNSKTSPR | |
| 331 | x | x | Y | Low | SHRSDGEFSPHSHYSDSDEAS |
| 334 | x | Y | High | SDGEFSPHSHYSDSDEAS | |
| 339 | x | x | N* | No | SDGEFSPHSHYSDSDEAS |
| 345 | x | x | N* | N/A | SDGEFSPHSHYSDSDEAS |
| 347 | x | x | N* | N/A | SDGEFSPHSHYSDSDEAS |
a Position of the amino acid.
b Peptide found after Atoh1 overexpression only.
c Peptide found after Atoh1 and CK1 overexpression.
d Cross-species sequence comparison. Y, conserved; N*, non-conserved in one tested species; N**, non-conserved in two tested species; N, non-conserved.
e Cycloheximide-chase assay results shown in Fig. 4.
f Phosphorylated peptide sequence with the phosphorylated residue underlined.