FIGURE 3.

Membrane binding characteristics of WT-αS, NTAc-αS, and Endo-αS to SUVs containing cholesterol. A, binding curves comparing the affinity of αS to 1:1 Chol:POPS SUVs (open symbols) and 1:1 POPC:POPS SUVs (closed symbols). B, binding curves of αS to 1:1 POPC:Chol SUVs (open symbols) and POPC SUVs (closed symbols). The binding curves for SUVs (open symbols) shown in panel B could not be fitted using the solution to a simple quadratic equation (26) and the depicted lines are only a guide to the eye. All measurements were performed at room temperature in the presence of 10 mm Tris, 100 mm KCl buffered at pH 7.4. The error bars in all binding curves represent standard deviations from 3 independent measurements.