TABLE 1.
L50 values (μm) of monomeric αS for different lipid compositions
| Lipid and protein | POPS (100) | POPC:POPS (25:75) | POPC:POPS (50:50) | POPC:Chol (50:50) | Chol:POPS (50:50) | POPC:POPS (75:25) | POPC (100) |
|---|---|---|---|---|---|---|---|
| SUVs | |||||||
| Liposome diameter (nm) | 65 ± 4 | 63 ± 3 | 60 ± 6 | 69 ± 8 | 67 ± 7 | 58 ± 2 | 64 ± 3 |
| Zeta potential, ζ (mV) | −21.8 ± 1.3 | −19.8 ± 0.8 | −16.8 ± 1.5 | −1.8 ± 0.8 | −14.6 ± 2.5 | −12.6 ± 0.9 | −2.9 ± 0.6 |
| WT-αS | 57 ± 4 | 333 ± 7 | 294 ± 8 | >2500a | 539 ± 30 | 638 ± 11 | 2847 ± 137 |
| NTAc-αS | 54 ± 4 | 302 ± 10 | 310 ± 18 | >2500a | 455 ± 32 | 576 ± 15 | 1905 ± 65 |
| Endo-αS | 57 ± 5 | 257 ± 18 | 263 ± 16 | >2500a | 445 ± 31 | 524 ± 26 | 1967 ± 136 |
| LUVs | |||||||
| Liposome diameter (nm) | 126 ± 4 | NDb | 126 ± 3 | ND | ND | ND | 128 ± 4 |
| Zeta potential, ζ (mV) | −31.5 ± 1.2 | ND | −23.1 ± 1.1 | ND | ND | ND | −5.2 ± 0.3 |
| WT-αS | 572 ± 72 | ND | >2000a | ND | ND | ND | >2500a |
| NTAc-αS | 500 ± 32 | ND | >2000a | ND | ND | ND | >2500a |
| Endo-αS | 547 ± 21 | ND | >2000a | ND | ND | ND | >2500a |
a The binding data in these cases could not be fitted to the binding equation.
b ND, not determined.