FIGURE 2.

Borealin maintains hepatocyte cell size and genome ploidy. A, the 8-week-old Borealin^Δli mice showed normal livers and liver/body weight ratios of Borealin^Δli and control Borealin^f/f mice were measured. B, serum ALT and AST levels were measured in Borealin^f/f and Borealin^Δli mice. C, hematoxylin and eosin staining of liver sections of the 8-week-old mice. Hepatocyte diameters were quantified. Scale bars, 50 μm. D, the genome ploidy of hepatocytes was characterized by propidium iodide staining followed by fluorescence-activated cell sorting. E, karyotype analysis showed that Borealin deletion caused tetraploid cells in cultured tail-tip fibroblasts. F, H&E staining of liver sections from the 2-week-old mice. Scale bars: 50 μm.