FIGURE 4.

Borealin deletion does not lead to oval cell expansion in 3–8-month-old Borealin^Δli mice. A, Borealin^Δli mice showed normal liver/body weight ratios. B, serum ALT and AST levels were measured in Borealin^f/f and Borealin^Δli mice at the indicated ages. C, hematoxylin and eosin staining of liver sections from 3- and 8-month-old mice. Scale bars, 50 μm. D, hepatocyte diameters were quantified. E, IHC staining showed that there was no Sox9⁺ oval cell expansion. TUNEL staining showed that apoptosis of hepatocytes was undetectable.