FIGURE 5.

Borealin deficiency inhibits DDC-induced Sox9⁺HNF4α⁺ progenitor-like cell proliferation. A, 8-week-old Borealin^f/f and Borealin^Δli mice were treated with DDC diet. These mice were killed 3 weeks later. B, liver/body weight ratios of Borealin^Δli and control Borealin^f/f mice 3 weeks after DDC treatment were measured. C, serum ALT, AST, and T-BIL levels were measured in Borealin^f/f and Borealin^Δli mice after DDC treatment. D, apoptosis of hepatocytes was confirmed by TUNEL staining. E, Sirius Red staining in DDC-treated Borealin^Δli and Borealin^f/f livers. F, Sox9 and HNF4α immunofluorescent staining and Sox9⁺HNF4α⁺ progenitor-like cells were quantified. G, the mRNA levels of hepatocyte progenitor genes were measured by q-PCR in DDC-treated Borealin^Δli and Borealin^f/f livers. H, hepatocyte mitosis was determined by pH3S10 IHC staining. Ratio of pH3S10 positive hepatocytes was quantified. I, hepatocyte diameters were quantified.