FIGURE 6.
Valinomycin attenuates serotonin- and thapsigargin-induced vasoconstriction and [Ca2+]i increase. A and B, representative traces and data summary of 5-HT- (10 μm) and TG- (10 μm) evoked contractions in control aortic rings (in black; 5-HT = 100% ± 10.62, n = 9; and TG = 100% ± 16.07; n = 5), and in rings pre-incubated for 15 min with 500 nm valinomycin (+Val, in blue; 5-HT = 35.10% ± 4.67, n = 4 and TG = 5.57% ± 0.53, n = 3). C and D, representative traces and data summary of [Ca2+]i changes elicited by high KCl (70K, 70 mm) 10 μm 5-HT, and 2 μm TG and in control VSMC (5-HT = 100% ± 11.24, n = 93 and TG = 100% ± 12.64, n = 90) and in cells pre-incubated for 3 min with 100 nm valinomycin (5-HT = 3.68% ± 0.63, n = 89 and TG = 6.38% ± 1.95, n = 114). 10 mm Caf was applied at the end of each experiment as indicated. Values are the percentage of mean ± S.E. normalized to 70K responses. **, p < 0.01.