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. 2016 Aug 15;291(40):21171–21183. doi: 10.1074/jbc.M116.730218

TABLE 1.

Binding affinities of Imp5 with H3 tails by ITC

ND, not determined.

MBP-H3 tail(1–47)	K_D determined by ITC
	nm
MBP alone	ND
Wild type	57.2 (43.9, 89.2)^a
K14A/R17A/K18A/K23A/R26A/K27A	861.6 (661.8, 942.2)^a
K14A/R17A/K18A	26.1 (15.3, 52.3)^a
K23A/R26A/K27A	119.1 (74.0, 165.6)^a
V35A/K36A/K37A/P38A/H39A/R40A	198.1 (150.1, 241.5)^a
K14/R17/K18/K23/R26/K27/³⁵VKKPHR⁴⁰ to Ala	ND
H3 tail(1–28)	811.5 (664.4, 984.50)^a
H3 tail (no MBP tag)	60.4 (27.7, 92.4)^a
GST-H3 tail	47.1 (28.6, 76.9)^a

^a Numbers in parentheses represent the 68.3% confidence intervals on K_D as calculated using F-statistics and error-surface projection method.