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. 2016 Aug 18;291(40):21184–21194. doi: 10.1074/jbc.M116.751271

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Isolation of lipid A from A. pasteurianus LPS. A, TLC profile of the hydrophobic fraction obtained by weak acid hydrolysis followed by phase partitioning. TLC was performed using a solvent system consisting of CH₃Cl-CH₃OH-H₂O (65/25/4, v/v/v) and visualized using anisaldehyde-sulfuric acid reagent. B, ESI-TOF-MS spectra of the hydrophobic fraction. The spectra were obtained with a Mariner instrument in negative ion refractor mode. C, SDS-PAGE profile of the untreated (UT) or hydrophilic fraction after weak acid hydrolysis (AcOH). The components were separated in 15% gel and visualized by periodic acid-silver staining.