FIGURE 1.
Relationship between apparent affinity for mincle and total size of acyl groups attached to 6-OH groups of trehalose. Affinities for monoacylated (A) and diacylated (B) trehalose derivatives were determined in binding competition assays. Binding of radiolabeled mannose-conjugated serum albumin to biotin-tagged CRD from mincle immobilized in streptavidin-coated wells was detected in the presence of soluble competing ligands. KI values measured in the competition experiments closely approximate the KD values (11). Straight lines were fitted to the data by least-squares fitting. Results are reported as the means ± S.D. for n = 3–4 separate experiments, each performed in duplicate.