FIGURE 3.

Structure of the extended CRD from mincle. A, arrangement of disulfide bonds in C-type CRDs. The additional fourth disulfide bond in the extended CRD is highlighted in cyan. B, sequence comparisons of N-terminal extensions in CRDs. Sequences shown are from bovine mincle and human blood dendritic cell antigen 2, human dectin-2, macrophage C-type lectin (MCL, also designated dectin-3), human dendritic cell immunoreceptor (DCIR), and human hepatic lectin-1 (HHL-1, major subunit of the asialoglycoprotein receptor). C, overall structure of the extended CRD, showing bound trehalose and three Ca²⁺. Structures of the N-terminal extensions of mincle (D) and BDCA-2, PDB entry 4ZES (E) are shown. Proteins in schematic representations are shown in cyan for the extended CRD from mincle and green for BDCA-2. Disulfide bonds are highlighted in yellow, and other atoms are colored as in Fig. 2.