FIGURE 2.

Oligomeric forms of CAPS are in dynamic equilibrium. A, gel filtration of CAPS-Myc-His detected by Western blot of CN-PAGE. Input to the column is indicated. The column was calibrated by the elution of aldolase, ferritin, and thyroglobulin. 0.16–0.19 K_av fractions correspond to CAPS monomer. B, longer exposure of Western blot of 0.06–0.12 K_av fractions to detect oligomer. Gel analysis was conducted immediately after gel filtration. C, Western blot of 0.11 and 0.12 K_av fractions kept on ice for 24 h after gel filtration. Purified oligomer partially dissociated to monomer. Results shown are representative of two replicate studies.