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. 2016 Aug 15;291(40):21257–21270. doi: 10.1074/jbc.M116.728097

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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FIGURE 5. — CAPS dimers are enriched on liposomes containing Q-SNAREs and PI(4,5)P₂. A, diagram of flotation assay to separate bound from unbound CAPS-Myc-His in liposome binding studies. B, BN-PAGE Western blot of input (left), bound (right), and unbound (bottom) CAPS-Myc-His after flotation with either protein-free (PF) or PI(4,5)P₂- and Q-SNARE-containing liposomes. Liposomes contained PC/PI(4,5)P₂ (95:5). Results shown are representative of two studies. C, BN-PAGE Western blot of bound CAPS-Myc-His after flotation with either protein-free liposomes composed of PC/PS (85:15) or PC/PI(4,5)P₂ (95:5) or Q-SNARE-containing liposomes with the same lipid compositions as indicated. Liposomes were also extracted into 1% Triton X-100 to ensure complete removal of bound CAPS-Myc-His. Results shown are representative of two studies.