FIGURE 1.

KLF6 is required for macrophage motility. A, Lyz2^cre and Lyz2^cre:KLF6^fl/fl mice were subjected to thioglycollate-induced peritonitis. Responding inflammatory cells from the peritoneal cavity were harvested using sterile 1× PBS, and the number of macrophages (CD11b⁺/SiglecF⁻/Ly6G⁻) was quantified. This experiment was performed three times with similar results. B–E, BMDMs derived from Lyz2^cre and Lyz2^cre:KLF6^fl/fl mice (B and D) and RAW264.7 cells transfected with the pCI-neo-KLF6 or pCI-neo plasmids (C and E) were stimulated with 100 ng/ml LPS for 4 h. These cells were added to the upper chamber of migration or invasion tissue culture inserts and incubated for 18 h. The number of cells migrated (B and C) or invaded (D and E) across the membrane in control group (untreated Lyz2^cre BMDMs or RAW264.7 cells transfected with pCI-neo plasmids) was assigned as 100%, and -fold changes over this are indicated. Each experiment was performed three times with four replicates. The box plot represents the median with first and third quartiles, and whiskers represent minimum/maximum. Graphs represent mean ± S.D. *, p ≤ 0.05; N.S., not significant. p < 0.05 between indicated groups is considered significant.